Figure 4.

Contraction and relaxation of ventricular CM over three consecutive days of cell isolation from live cardiac tissue slices of the same heart. (A) Recordings of sarcomere shortening (in response to field stimulation at 0.5 Hz) over three consecutive days of CM isolation. (B) Degree of sarcomere shortening (%) in ventricular CM over three consecutive days. (C) Maximum velocity of contraction and relaxation in ventricular CM over three consecutive days of CM isolation; n = 4–8 hearts, 150–300 CM/day of isolation. (B,C), each color represents data from one heart. Within each colored area, the open circles represent the average of all cells recorded from the same heart.