Antibody (Clone 13B8.2, CN: COIM0704) was purchased from Fisher Scientific (Fair Lawn, NJ, USA)
APTES slide	APTES, 3-Aminopropyl Trimethoxysilane treated slide
ASSURED	Affordable, sensitive, specific, user-friendly, rapid and robust, equipment-free, and deliverable
BSA	lyophilized bovine serum albumin (BSA, CN a2153). A small stable protein of 607 amino acids, it is used as a blocker to help antibodies find antigens by binding to non-specific binding sites
DMSO	Dimethyl sulfoxide is polar organic solvent that is miscible with water. We mixed the GMBS with DMSO so that it can be further cut and soluble in water. We can then apply to our substrate. So that GMBS can adhere to the substrates, we have a double bond to the surface
DPBS	D phosphate-buffered saline solution
DSA	Double sided adhesive tape. Forms the channel and binds the top and bottom layer in the micro module
EDTA	Ethylenediaminetetraacetic acid; prevents the coagulation of blood cells by absorbing mental ions to prevent binding of beads and blood
GMBS	N-γ-Maleimidobutyryloxy succinimide ester; this is a chemical that is a cross linker from amino and sulfhydryl groups. It comes as a solid and is water insoluble. Therefore, must be mixed with DMSO or DMF [3]. We use DMSO
HC	Hemocytometer count
HIV	Human immunodeficiency virus
Maleimide Activated Neutravidin	This version can bond directly without using GMBS to cross link to slide
MC	Microscope count
MPS	(3-Mercaptopropyl) trimethoxysilane (3-MPS, CN: 175617), b. This compound is an adhesive; it has four hydrogen acceptor molecules and one donator
It is the first layer to adhere to a glass slide that has donor atoms
Neutravidin protein	This protein is used to attach to the GMBS and the anti-CD4 antibody. Ka = 1015 M−1
NucBlue	This stain is best for live and dead cells. It is from Invitrogen and similar to DAPI
PMMA	Polymethymetacrylate sheet. It is an acrylic sheet that can be easily cut with a laser cutter
POC	Point-of-care
WHO	World Health Organization
Other items used were: anti-CD4 beads, absolute alcohol: ethanol 200 proof, OH−, and glass slides