Figure 1.

Flexcell Bioflex and Tissue Train models. (A) Image of a 6-well bioflex 6-well culture plate with a flexible membrane bottom and a schematic showing a 2D monolayer of cells grown in the plate. (B) Image of a 6-well tissue train culture plate placed on a loading post with the groove in which 3D collagen gels are made. A schematic showing the application of uniaxial strain on a linear fibroblast-seeded 3D collagen gel through the application of vacuum pressure underneath the loading post. (C) A schematic showing the production of fibroblast-seeded 3D linear gels in the Tissue train system before the application of strain. Adapted from Garvin et al. [24] and Flexcellint^®.