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. 2022 Jan 6;11(2):185. doi: 10.3390/cells11020185

Figure 3.

Figure 3

The response of lung fibroblasts to fibrotic collagen-I 3D microenvironment. Human fetal lung 1 (HFL1) fibroblasts were embedded in 3D collagen type-I gels of 0.4 mg/mL and 2.0 mg/mL concentration and cultured for 24 h. Representative confocal image of HFL1s in (A) 0.4 mg/mL and (B) 2.0 mg/mL collagen-1 gels after culturing for 24 h and staining for non-muscle myosin IIB (red) and F-actin (green). (C) Average total cell counts in 0.4 mg/mL and 2.0 mg/mL collagen-I gels. (D) Percentage cell numbers of spindle and rounded shaped cells in 0.4 mg/mL and 2.0 mg/mL collagen-I gels. (E) Percentage positive pixel count per total number of pixels in images of 0.4 mg/mL and 2.0 mg/mL collagen-I gels. The concentration of (F) IL-6 and (G) IL-8 cytokines released from HFL1 fibroblasts in 0.4 mg/mL and 2.0 mg/mL collagen-1 gels. (G) Percentage cell viability of HFL fibroblasts in 0.4 mg/mL and 2.0 mg/mL collagen-I gels. Mean ± SEM indicated for 3 technical replicates, n = 6. * p < 0.05.