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. 2021 Dec 25;12(1):30. doi: 10.3390/biom12010030

Figure 1.

Figure 1

Propyl gallate (PG) and epigallocatechin gallate (EGCG) decrease rRNA transcription and H3K36me2 levels in the rRNA gene promoter through KDM2A. (A) MCF-7 cells transfected with control siRNA or KDM2A siRNA were treated with the indicated concentrations of gallic acid (GA), PG or EGCG for 4 h. Total RNA was extracted, and the levels of rRNA transcripts (pre-rRNA) (left panel) and KDM2A mRNA (right panel) were determined using quantitative real-time PCRs (qRT-PCR). The results are shown as the fold change in relation to cells treated with control siRNA in the absence of compounds. (B) MCF-7 cells transfected with control siRNA or KDM2A siRNA were treated with 50 μM GA, PG or EGCG for 4 h. The levels of H3K36me2, H3K36me3, and KDM2A in the rRNA gene promoter were analyzed by ChIP assay. The results are shown the fold change in relation to cells treated with control siRNA in the absence of compounds. All experiments were performed more than three times, and the mean values with standard deviations are shown. * p < 0.05.