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. 2022 Jan 8;11(2):206. doi: 10.3390/cells11020206

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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The impact of CerS1 and CerS5 silencing on the content of bioactive lipids, acyl-carnitines, and the protein expression of CPT1B in gastrocnemius muscle of HFD-fed mice: Panel (A)—total content of LCACoA; Panel (B)—total content of DAG; Panel (C)—total content of TAG; Panel (D)—total Cer content; Panel (E)—total content of acyl-carnitines, Panel (F)—protein of CPT1B. Values are mean ± SD (n = 8 per group); a—p < 0.05 vs. LFD; b—p < 0.05 vs. HFD; significance by ANOVA; LFD-low-fat diet, HFD—high-fat diet. The observed molecular weight of indicated proteins is different to the theoretical one, as stated by the antibody manufacturer.

The impact of CerS1 and CerS5 silencing on the content of bioactive lipids, acyl-carnitines, and the protein expression of CPT1B in gastrocnemius muscle of HFD-fed mice: Panel (A)—total content of LCACoA; Panel (B)—total content of DAG; Panel (C)—total content of TAG; Panel (D)—total Cer content; Panel (E)—total content of acyl-carnitines, Panel (F)—protein of CPT1B. Values are mean ± SD (n = 8 per group); a—p < 0.05 vs. LFD; b—p < 0.05 vs. HFD; significance by ANOVA; LFD-low-fat diet, HFD—high-fat diet. The observed molecular weight of indicated proteins is different to the theoretical one, as stated by the antibody manufacturer.