Table 2.
Impact of CerS1 and CerS5 gene silencing on the content of individual long-chain acyl-CoA’s in mouse gastrocnemius muscle of HFD-fed animals. Values are mean pmol/mg of tissue ± SD; n = 8 per group; a—p < 0.05 vs. LFD.
| C14:0-CoA | C16:0-CoA | C16:1-CoA | C18:0-CoA | C18:1-CoA | C18:2-CoA | C20:0-CoA | C22:0-CoA | C24:0-CoA | C24:1-CoA | |
|---|---|---|---|---|---|---|---|---|---|---|
| LFD | 0.11 ± 0.03 | 0.85 ± 0.10 | 0.78 ± 0.11 | 0.73 ± 0.08 | 2.59 ± 0.30 | 1.71 ± 0.29 | 0.017 ± 0.003 | 0.022 ± 0.003 | 0.028 ± 0.004 | 0.016 ± 0.005 |
| HFD | 0.20 ± 0.03 a | 0.99 ± 0.13 | 0.68 ± 0.07 | 1.20 ± 0.15 a | 2.69 ± 0.43 | 1.96 ± 0.27 | 0.019 ± 0.004 | 0.033 ± 0.005 a | 0.031 ± 0.005 | 0.009 ± 0.001 a |
| HFD(-CerS1) | 0.21 ± 0.02 a | 1.03 ± 0.23 | 0.71 ± 0.08 | 1.18 ± 0.16 a | 2.61 ± 0.32 | 2.07 ± 0.27 | 0.019 ± 0.002 | 0.035 ± 0.004 a | 0.032 ± 0.003 | 0.009 ± 0.002 a |
| HFD(-CerS5) | 0.21 ± 0.06 a | 0.99 ± 0.39 | 0.70 ± 0.08 | 1.18 ± 0.17 a | 2.67 ± 0.32 | 2.20 ± 0.32 a | 0.020 ± 0.002 | 0.035 ± 0.003 a | 0.031 ± 0.003 | 0.010 ± 0.002 a |