Table 4.
Impact of CerS1 and CerS5 gene silencing on the content of individual sphingolipids in mouse gastrocnemius muscle of HFD-fed animals. Values are mean pmol/mg of tissue ±SD; n = 8 per group; a—p < 0.05 vs. LFD; b—p < 0.05 vs. HFD.
| C14:0-Cer | C16:0-Cer | C18:0-Cer | C18:1-Cer | C20:0-Cer | C22:0-Cer | C24:0-Cer | C24:1-Cer | Sph | SPA | S1P | |
|---|---|---|---|---|---|---|---|---|---|---|---|
| LFD | 0.029 ± 0.002 | 1.94 ± 0.22 | 16.9 ± 1.4 | 0.56 ± 0.08 | 0.27 ± 0.04 | 0.77 ± 0.12 | 1.23 ± 0.14 | 3.8 ± 0.4 | 0.38 ± 0.05 | 0.2 ± 0.02 | 0.04 ± 0.01 |
| HFD | 0.034 ± 0.008 | 3.20 ± 0.35 a | 30.3 ± 3.4 a | 0.67 ± 0.05 a | 0.57 ± 0.09 a | 1.45 ± 0.21 a | 2.55 ± 0.32 a | 5.1 ± 0.6 a | 0.54 ± 0.12 a | 0.3 ± 0.05 a | 0.27 ± 0.01 a |
| HFD(-CerS1) | 0.033 ± 0.005 | 2.80 ± 0.36 ab | 16.5 ± 0.9 b | 0.40 ± 0.05 ab | 0.56 ± 0.09 a | 1.49 ± 0.19 a | 2.05 ± 0.24 ab | 4.2 ± 0.4 b | 0.55 ± 0.11 a | 0.3 ± 0.04 a | 0.05 ± 0.01 b |
| HFD(-CerS5) | 0.038 ± 0.010 | 1.99 ± 0.18 b | 26.5 ± 2.6 ab | 0.48 ± 0.07 b | 0.51 ± 0.07 a | 1.40 ± 0.15 a | 2.45 ± 0.27 a | 4.1 ± 0.5 b | 0.84 ± 0.14 ab | 0.3 ± 0.03 a | 0.31 ± 0.01 a |