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. 2021 Dec 27;12(1):34. doi: 10.3390/biom12010034

Figure 5.

Figure 5

RTP801 silencing in the R6/1 mouse hippocampal neurons reduces the levels of the inflammasome components. (a) Immunoblottings for NLRP1, cleaved NLRP1, procaspase 1, cleaved caspase 1, ASC/TMS1, and GFP as loading control for transduced neurons in the dorsal hippocampus of 4.5-month-old WT shCt, WT shRTP801, R6/1 shCt, and R6/1 shRTP801 mice. (b–f) Densitometric quantification of NRLP1 (treatment effect: F(1, 26)  = 24.89, p  < 0.0001; interaction: F(1, 26)  =  1.175, p  =  0.2883), cleaved NLRP1 (treatment effect: F(1, 29)  =  44.18, p < 0.0001; genotype effect: F(1, 29)  =  15.32, p = 0.0005; interaction: F(1, 29)  =  6.588, P  =  0.0157), ASC/TMS1 (treatment effect: F(1, 31)  =  40.72, p < 0.0001; genotype effect: F(1, 31)  =  17.85, p = 0.0002; interaction: F(1, 31)  =  15.91, p  =  0.0004), procaspase 1 (treatment effect: F(1, 30)  = 26.06, p   < 0.0001; interaction: F(1, 30)  =  10.82, p  =  0.0026) and cleaved caspase 1 (treatment effect: F(1, 30)  = 25.57, p   < 0.0001; interaction: F(1, 30)  =  5.551, p  =  0.0252) as in (a) for the hippocampus. Densitometric quantification of all proteins is expressed as the mean  ±  SEM. All data were analyzed by two-way ANOVA followed by Bonferroni’s post hoc test. * p  <  0.05, ** p  <  0.01, and *** p  <  0.001.