Figure 3.

Back-table fluorescent tumor labeling of patient with tumor-positive circumferential resection margin (CRM) using bevacizumab-IRDye800. A black pin at the location shows increased fluorescence, to enable accurate correlation with histology ((A), orange arrow). Fluorescence imaging of 2 corresponding tissue slices (B) and further microscopic fluorescence imaging and histologic correlation (C), with orange arrows indicating the location of tumor-positive CRM. High fluorescence signals were observed at CRM of tissue slice 1, containing tumor-positive CRM, whereas low fluorescence signals were observed in nontumor tissue slice 2, corresponding to microscopy results. FFPE: formalin-fixed paraffin-embedded; HE: hematoxylin–eosin; N: nontumor; T: tumor. Adapted from de Jongh et al. [151].