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. 2022 Jan 6;14(2):276. doi: 10.3390/cancers14020276

Figure 1.

Figure 1

Transcriptomic analysis of 32 canine MM samples. (A) NMF clustering of expression data identified two subgroups that overlap with SV content. The first subgroup is characterized by the expression of immune response and cytokine-mediated signaling pathways and a low SV content, whereas the second subgroup is characterized by the expression of melanin metabolic processes and pigmentation pathways and is enriched in SV as well as focal amplifications of MDM2, CDK4, or CFA30:17Mb region. (B) Expression ratio of proinflammatory cytokines (IFN-γ, IL-1A, IL-1B, and IL-2) and immunosuppressive molecules (IL-10, IL-11, and TGFB1) significantly differs between the two groups (p = 0.03, Student test). (C) Expression of immune checkpoint genes or known oncogenes according to the transcriptomic classification. The following immune checkpoint genes CTLA4, LAG3, TIM3, and IL2RG are overexpressed in group 1, while the oncogenes MITF, TERT, KIT, and POM121 are overexpressed in group 2.