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. 2022 Jan 6;14(2):276. doi: 10.3390/cancers14020276

Figure 3.

Figure 3

Expression data of the CFA 10 and CFA30 candidate genes at mRNA and protein levels and impact on cell proliferation. (A). For the CFA 10 genes (MDM2, CDK4) and CFA 30 genes (GABPB1, TRPM7, SPPL2A and USP8), expression was defined by RT-qPCR and was significantly correlated to copy number in tumor tissue (n = 46). (B). Western blot analysis in six canine MM cell lines showing a higher amount of protein in cell lines having high copy numbers of CDK4 and MDM2. (C). Cell proliferation assay (Bear cell line) showing the effect of the knockdown (siRNA) of candidate oncogenes from CFA 30 and CDK4 on cell survival. The knockdown of CDK4, TRPM7, GABPB1, and SPPL2A had an impact on cell proliferation in comparison with the control siRNA (t-test, with * corresponding to p-values <0.05 and ** to p-values <0.01).