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. 2022 Jan 10;14(2):323. doi: 10.3390/cancers14020323

Figure 2.

Figure 2

Intracellular cytokine production in the continues presence of NAM. NAM induces higher production of TNF and IL2 in both CD4+ and CD8 T cells and higher production of IFN in CD8+ T cells. (A) Time course shown for average across four flow cytometry experiments, with error bars indicating standard deviation, and statistical significance between treated and untreated indicated by * as determined using the Holm-Sidak method (α = 0.05). Additional statistics performed on (B) combined data when compared to untreated only for each time point, on days five to nine across n = 5 in vitro stimulation experiments, mann-whitney test (p < 0.05 indicated by *), and (C) representative plots showing difference in granzyme B and TNFα expression patterns. (D) Cumulative cytokine expression of total CD8+ T cells, and (D) inset IR expressing CD8+ T cells as scored for representative experiment shown in 2A, showing significance by chi-squared analysis (* p < 0.05). Data points marked in (B) as ‘X’ were obtained from mass cytometry analysis, with remainder from flow cytometry.