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. 2022 Jan 11;11(2):229. doi: 10.3390/cells11020229

Figure 6.

Figure 6

The MyD88–JAK2/TYK2–STAT-3 axis is involved in eHSP90α-induced proangiogenic activity of M2-type macrophages. (A) IHC of CD31 from the tumors formed by Panc 02 cells alone or Panc 02 cells mixed with PBS-, LPS-, or rHSP90α-treated BMDMs. (B) mRNA levels of VEGF, platelet-derived growth factor (PDGF), and bFGF in the BMDMs treated for 24 h with control medium (Ctrl), Endo-CM, EndoMT-CM, or EndoMT-CM plus control IgG or anti-HSP90α antibody. The mean ± SD of qPCR data were obtained from three independent experiments. @ p < 0.01 when compared with “Ctrl” group. # p < 0.01 when compared with “EndoMT-CM + control IgG” group. (C) mRNA levels of VEGF, PDGF, and bFGF in the BMDMs treated for 24 h with PBS, rHSP90α, or rHSP90α plus control IgG or anti-HSP90α antibody. The qPCR data shown are the mean ± SD of three independent experiments. @ p < 0.01 when compared with “PBS” group. # p < 0.01 when compared with “rHSP90α + control IgG” group. (D) mRNA levels of VEGF, PDGF, and bFGF in the THP-1-derived macrophages treated for 24 h with PBS, rHSP90α, or rHSP90α plus control IgG or anti-CD91 or TLR4 antibody. The representative RT-PCR data of three independent experiments are shown. (E) mRNA levels of VEGF, PDGF, and bFGF in the THP-1-derived macrophages treated for 24 h with PBS, rHSP90α, or rHSP90α plus DMSO, IKKi, JSI-124, or JAKi. The RT-PCR data shown are the representative of three independent experiments. (F) mRNA levels of VEGF, PDGF, and bFGF in the BMDMs treated for 24 h with control medium (Ctrl), Endo-CM, EndoMT-CM, or EndoMT-CM plus DMSO, JAKi, or JSI-124. The qPCR data shown are the mean ± SD of three independent experiments. @ p < 0.01 when compared with “Ctrl” group. # p < 0.01 when compared with “EndoMT-CM + DMSO” group. (G) mRNA levels of VEGF, PDGF, and bFGF in the BMDMs treated for 24 h with PBS, rHSP90α, or rHSP90α plus DMSO, JAKi, or JSI-124. The qPCR data shown are the mean ± SD of three independent experiments. @ p < 0.01 when compared with “PBS” group. # p < 0.01 when compared with “rHSP90α + DMSO” group.