Figure 5.

Mutations of the PI4Kα1 complex induce sporophytic phenotypes. A, Opened siliques of self-fertilized Col-0, hyc2-2 and hyc2-3 heterozygous mutant plants. White arrowheads indicate aborted seeds. B, Percentage of aborted seeds in Col-0, hyc2-2^+/−, hyc2-3^+/−, hyc2-2^−/− HYC2_pro:HYC2-mCITRINE (insertion no. 10), and hyc2-2^−/− HYC2_pro:HYC2-2xmCHERRY (insertion no. 11) siliques. The number of seeds counted is superior to 250 for each genotype. Statistics used chi-square test. n.s., nonsignificant; ***P < 0.001. C, Cleared seeds from hyc2-2 and hyc2-3 heterozygous mutant plants. White arrowheads indicate globular embryos that have stopped development. D, Twenty-seven-day-old Col-0, npg1-2^+/−, npgr1^−/−, npgr2-2^−/−, npg1-2^+/− npgr1^−/−, npgr1^−/− npgr2-2^−/−, and npg1-2^+/− npgr1^−/− npgr2-2^−/− plants. Scale bar: 2 cm. E, Forty-one-day-old Col-0, npg1-2^+/−, npgr1^−/−, npgr2-2^−/−, npg1-2^+/− npgr1^−/−, npgr1^−/− npgr2-2^−/−, and npg1-2^+/− npgr1^−/− npgr2-2^−/− plants. Scale bar: 2 cm. F, Thirty-eight-day-old Col-0 and plants expressing microRNA2 and microRNA3 against PI4Kα1. Scale bar: 2 cm.