Figure 3.

Camostat could not potently inhibit the replication of Omicron variant in VeroE6/TMPRSS2 cells. (A) Bafilomycin A1 inhibited the Omicron and Delta replication in VeroE6/TMPRSS2 cells. (B) Chloroquine inhibited the Omicron and Delta replication in VeroE6/TMPRSS2 cells. (C) Camostat could potently inhibit Delta variant but not Omicron variant replication in VeroE6/TMPRSS2 cells. Cells were pretreated by the indicated bafilomycin A1, chloroquine or camostat before viral infection, 1000 TCID₅₀ of virus was added to cells for infection and then viral RNA copies in cell lysate were measured at 8 hpi by RT-qPCR. Viral RNA (%) was normalized to the viral RNA copy of untreated virus. ** indicates P < 0.01 and *** indicates P < 0.001 when compared with untreated virus. Data are presented as mean ± SD from three independent biological samples.