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. 2022 Jan 18;29(1):328–341. doi: 10.1080/10717544.2022.2027046

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© 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Relative cell proliferation of (A) keratinocyte (HaCaT) and (B) human follicle dermal papilla (hDP) cells after incubation with bimatoprost (BIM) in ethanol, BIM topical formulations (BIM–TFs), and vehicle (BIM–TF#5 diluted to BIM concentrations of 0.01, 0.1, 0.5, 1, 2.5, 5, and 10 µM BIM), and 5% minoxidil in ethanol diluted to a concentration equivalent to that of 10 µM minoxidil. **p<.01, ***p<.001 compared to vehicle (BIM–TF#5); ^#p<.05, ^##p<.01, ^###p<.001 compared to BIM in ethanol; ^$p<.05, ^$$$p<.001 compared to BIM–TF#1; ^&&p<.01, ^&&&p<.001 compared to BIM–TF#5. Relative scratch wound recovery of hDP cells after incubation with BIM in ethanol, BIM–TF#1, BIM–TF#5, and vehicle of BIM–TF#5 diluted to a concentration equivalent to that of 5 μM BIM, and minoxidil in ethanol diluted to a concentration equivalent to that of 10 µM minoxidil. (C) Time–course curve of relative scratch wound recovery area of hDP cells. **p<.01, ***p<.001 compared to control (untreated); ^###p<.001 compared to vehicle (BIM–TF#5); ^$p<.05, ^$$$p<.001 compared to BIM in ethanol (5 µM); ^&&p<.01, ^&&&p<.001 compared to BIM–TF#1 (5 µM); ^@@p<.01 compared to BIM–TF#5 (5 µM). Values represent means ± SDs (n = 6). (D) Representative microscope images of scratch wounds of hDP cells. Scale bar = 200 µm.