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LDL oxidation. Three LDL fractions were assessed: LDL freshly isolated from EDTA-plasma (EDTA-LDL),) LDL further purified by dialysis (nLDL), and nLDL treated with CuSO4 for 24 h (oxLDL). The level of oxidation was measured by (A) ELISA (n = 6), (B) absorbance at 234 nm (n = 4), and (C) gel electrophoretic mobility (n = 3), as described in Methods, with n referring to a number of separate LDL preparations. In (C), an exemplary gel is shown. t-test mean +/− SD with * p < 0.05.
