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. 2022 Jan 8;11(2):204. doi: 10.3390/cells11020204

Figure 4.

Figure 4

Effect of nLDL and oxLDL on LDLR and LOX-1 expression. (A–D) Total RNA was harvested from HUAECs treated with either nLDL or oxLDL, and analyzed for LDLR and LOX-1 mRNA expression by reverse transcription-PCR. The results are presented in relation to the expression levels in untreated control cells. In A and B, HUAECs were incubated in the presence or absence of 100 µg/mL of nLDL or oxLDL for the times indicated (n = 6). In C and D, HUAECs were incubated for 12 h with either nLDL or oxLDL at doses indicated (n = 6). (E,F): Total protein was extracted from HUAECs incubated with 100 µg/mL of either nLDL or oxLDL for 24 h or (F) 7 h, and expression of target proteins was measured by Western blotting. Asterisks and hash signs represent a significant difference compared to control cells and nLDL-treated cells, respectively. t-test mean +/− SD with vs. 1 h * p < 0.05 and # vs. nLDL p < 0.005 ns = not significant.