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. 2021 Dec 28;13(1):82. doi: 10.3390/genes13010082

Figure 4.

Figure 4

(a) Proliferation rate of M1 (ZZ genotype) PGC line on Day 1, Day 2 and Day 3. M1-C: non-inhibited, M1-5P: gga-miR-302b-5P inhibition, M1-3P: gga-miR-302b-3P inhibition, M1-5P/3P: gga-miR-302b-5P and gga-miR-302b-3P inhibition. (bd) Immunostaining of M1 PGCs after three days of cultivation in different culture conditions. The immunostaining was achieved with SSEA-1 (red), CVH (green) and TO-PRO™-3 for nuclear staining (blue) (p < 0.01 **, p < 0.001 ***).