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. 2022 Jan 15;11(2):294. doi: 10.3390/cells11020294

Figure 1.

Figure 1

Senescence analysis in the U87-MG glioblastoma cell line. Cytofluorometric analysis of Spider-β galactosidase (spider-βGal), common senescence marker, in the U87-MG glioma cell line after 24 h of treatment with etoposide (6 μM) and 5 days resting in the presence or absence of 18 ng/mL of rhLAV-BPIFB4 for the last 48 h. The treatment with etoposide induced senescence as shown in (A) (middle graph); after the treatment with 18 ng/mL of rhLAV-BPIFB4 the spider-βGal values decreased (right graph in (A)). (B) Bar graph reporting the mean percentage values ± SD of β galactosidase viable cells from 3 independent experiments. (C) Cytofluorimetric analysis of the HLA-E expression on U87-MG cells’ surfaces in the control and in ETP-treated cells with or without rhLAV-BPIFB4 for the 5 days resting as indicated in Section 2. The bar graphs report the mean ± SD of the percentage of positive cells in the different conditions. Statistical analysis by two-way ANOVA with Tukey’s test for multiple comparison was conducted. (** p < 0.001, *** p < 0.0001, **** p < 0.00001).