Figure 1.

SOS4 is important for shoot and root development. A, Upper panel: Scheme of the architecture of SOS4. Arrows indicate position of EMS mutations, the triangle shows the location of the T-DNA insertion. Lower panel: expression analysis of sos4 alleles bearing T-DNA insertions. Data are the mean of three biological replicates ± SD (each comprising ≥20). B, 3-week-old plants grown on soil in 16-h light (120 µmol photons m⁻² s⁻¹ at 21°C), 8 h dark (at 18°C) cycles. C, 10-d-old seedlings grown on sterile culture medium under the same conditions as (B). D, Roots of 7-d-old seedlings grown on sterile culture medium under the same conditions as (B). E, 6-week-old plants grown in a rhizobox in 8-h light (120 µmol photons m⁻² s⁻¹ at 21°C), 16-h dark (at 18°C) cycles. F, 2-week-old plants in a rhizobox under the same conditions as (B).