Figure 7.

HPLC profiling of vitamin B₆ content of plants grown in rhizoboxes. A, Left side, a scheme of the rhizobox system used. The soil and a membrane are layered between two perspex plates. Seeds are sown on the top at the membrane side and do not touch the soil. Right side, total vitamin B₆ content of shoots and roots of 2-week-old plants grown in rhizoboxes in 16-h light (120 µmol photons m⁻² s⁻¹ at 21°C), 8-h dark (at 18°C) cycles. The key indicates the color coding used for each line. B, Individual B₆ vitamer content in shoots of plants grown as in (A). The individual vitamers are pyridoxal 5′-phosphate (PLP), pyridoxamine 5′-phosphate (PMP), pyridoxal (PL), pyridoxamine (PM), and pyridoxine (PN). To aid visualization PM and PN contents are shown as an inset on a smaller scale. C, Individual B₆ vitamer content in roots of plants grown as in (A). Vitamers as in (B). D, Individual B₆ vitamer content in shoots of 2-week-old plants grown in rhizoboxes in 16-h light (20 µmol photons m⁻² s⁻¹ at 21°C), 8-h dark (at 18°C) cycles. Vitamers and inset as in (B). E, Individual B₆ vitamer content in roots of plants grown as in (D). Vitamers as in (B). The data represent the mean ± sd of three biological replicates (each comprising ≥20 plants collected from three rhizoboxes). Asterisks indicate significance by one-way ANOVA test (*P-value < 0.05, **P-value < 0.01, ***P-value < 0.001). A separate ANOVA was used for each line in comparison with the wild type.