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. 2022 Jan 14;149(2):dev199988. doi: 10.1242/dev.199988

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Fig. 3. — C2CD6 is required for in vitro fertilization and sperm hyperactivation. (A) In vitro fertilization. CD1 cumulus-oocyte complexes were incubated with C2cd6^+/− or C2cd6^−/− sperm. Fertilization rate is the percentage of oocytes inseminated that develop into two-cell embryos after 24 h of incubation. A representative image for each treatment is shown. ****P<0.0001. (B) Percentage of two-cell embryos that develop to the blastocyst stage after culture in KSOM media. A representative image for each treatment is shown. ***P<0.001. (C) Percentage of motile sperm immediately after swim-out in TYH medium (T0) and after 60 min of incubation in capacitating conditions in TYH medium (T60). Sperm motility was analyzed by computer-assisted sperm analysis. (D) Percentage of the motile sperm displaying progressive motility at T0 and at T60 of incubation in capacitating conditions. (E) Percentage of the motile sperm displaying hyperactive motility at T0 and at T60 of incubation in capacitating conditions. ns, not statistically significant, **P<0.01. Unpaired Student's t-tests were performed for statistical analysis. n=5 or 6.