Table 1.
Known APC subunits identified through AP/MS from maize callus, leaf division zone (DZ), and expansion zone (EZ) and in Arabidopsis using SAMBA-GSrhino as bait
| Protein ID | At ortholog | Description | In maize callus |
In maize leaf DZ | In maize leaf EZ | In Arabidopsis | |
|---|---|---|---|---|---|---|---|
| −log(p) | Difference | ||||||
| GRMZM2G157878 | AT1G32310 | SAMBA | 7.05 | 11.07 | x | x | x |
| GRMZM2G392710 | AT2G20000 | APC3b | 6.6 | 10.11 | x | x | x |
| GRMZM2G089296 | AT2G39090 | APC7 | 7.08 | 9.76 | x | x | x |
| GRMZM2G170591 | AT3G48150 | APC8a | 6.95 | 10.16 | x | x | x |
| GRMZM2G168886 | AT2G04660 | APC2 | 7.55 | 8.87 | x | x | x |
| GRMZM2G053766 | AT4G21530 | APC4 | 9.27 | 9.5 | x | x | x |
| GRMZM2G053980 | AT5G05560 | APC1 | 8.72 | 7.93 | x | x | x |
| GRMZM2G012220 | AT5G05560 | APC1 | 6.19 | 7.81 | x | x | x |
| GRMZM2G431251 | AT1G06590 | APC5 | 6.39 | 7.51 | x | x | x |
| GRMZM2G054247 | AT2G18290 | APC10a | 6.62 | 7.12 | x | x | x |
| GRMZM2G354696 | AT5G05560 | APC1 | 4.67 | 6.48 | x | x | x |
| GRMZM2G020201 | AT5G63135 | APC15b | 5.05 | 4.27 | x | x | |
| GRMZM2G064005 | AT4G11920 | CCS52A2 | 3.91 | 3.92 | x | x | x |
| GRMZM2G159427 | AT4G22910 | CCS52A1 | 4.47 | 3.46 | x | x | |
| GRMZM2G166684 | AT1G78770 | APC6b | 4.83 | 3.02 | x | x | x |
| GRMZM2G147603 | AT1G78770 | APC6a | NA | NA | x | x | |
| GRMZM5G821639 | AT5G13840 | CCS52B | NA | NA | x | ||
Protein identifications are based on significant enrichment compared to the non-SAMBA-related GIF2 and GIF3 control purifications grouped together (n = 3). Arabidopsis orthologs were determined using PLAZA 3.0 based on best-hit Interparalog families (BHIF) (Proost et al., 2015). Difference: log2 (LFQ ratio [SAMBA/GIF]) presents the log2 fold change in protein abundance in GIF samples compared to SAMBA samples. Significance of enrichment was calculated using permutation-based FDR-corrected t test (FDR = 0.01, S0 = 1). x indicates that a subunit was identified in this sample; NA = not applicable.