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. 2022 Jan 13;11(2):210. doi: 10.3390/foods11020210

Table 1.

Primers used for PCR assay.

Gene Target Length bp Primer Sequences (5′ → 3′) Reference
Internal transcribed Spacer (ITS) 420–825 ITS1 (F) 5′TCCGTAGGTGAACCTGCGG3′ [22]
ITS4 (R) 5′TCCTCCGCTTATTGATATGC3′
β-tubulin (BenA) 1125 β-tub 2a (F) 5′GGTAACCAAATCGGTGCTTTC3′ [22]
β-tub 2b (R) 5′ACCCTCAGTGTAGTGACCCTTGGC3′ [23]
Calmodulin (CaM) 543 Cmd5 (F) 5′-CCGAGTACAAGGAGGCCTTC-3′ [23]
Cmd6 (R) 5′-CCGATAGAGGTCATAACGTGG-3′

Abbreviation: F: Forward, R: Reverse. BenA means B-tubulin and CaM mean calmodulin.