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. Author manuscript; available in PMC: 2023 Feb 1.
Published in final edited form as: J Neurosci Methods. 2021 Dec 7;367:109437. doi: 10.1016/j.jneumeth.2021.109437

Figure 4:

Figure 4:

ACAT activity remains severely inhibited in cells treated with F12511 or with Nanoparticle F for several hours after F12511 removal from media. A.) Human neuronal cells (SH-SY5Y), human microglial cells (HMC3), mouse neuronal cells (N2a), and mouse microglial cells (N9) were either treated for 2 hours with 0.5μM EtOH (control), F12511 alone, or Nanoparticle F. After treatment, the media was removed, cells were washed twice with drug-free media kept at 37°C, given fresh drug-free media, and put back in the 37°C incubator with 5% CO2 for 0-, 2-, 4-, and 8-hour(s). The cells were then pulsed with 3H-oleate and ACAT activity was determined by cholesteryl ester formation in intact cells. Two-way ANOVA was completed, and reported results are relative to the control. N.S. not significant; p<0.001 ***, p<0.01 **, p<0.05 *. B.) Mouse microglial cells (N9) were treated with DMSO (control) or with 0.5μM F12511 in DMSO for 4 hours. After treatment, cells were washed twice with drug-free media at 37°C, given fresh drug-free media, and placed back in the 37°C incubator with 5% CO2 for up to 4 hours. At time indicated, cells were harvested, and the mixed liposomal ACAT activity assay in vitro was performed.