Figure 6.

Competitive fitness analysis for six POS_Pep clones. The respective plasmids were cloned into the E. coli B REL606 and REL607 ancestor backgrounds that produce red and pink colonies, respectively, on TA indicator plates (see Methods). The competitor in each experiment was the NS_Pep3 clone. (a,b) represent the experiments for swapped backgrounds to ensure that there are no background-specific effects. The symbol # represents missing data. At least four replicates were used for each experiment. Whiskers represent the SEM.