Fig. 2.

The effect of SARS-CoV-2 SP (50 ng/mL) on expression of key pro- and anti- proliferative molecules in LNCaP cells. Statistically significant results are indicated by asterisk (*) (p < 0.05). A Comparison of mRNA expression levels in pro- and anti-proliferative molecules in LNCaP cells. Pro-proliferative molecules included cyclin B, cyclin D, cyclin E, and CDK4. Anti-proliferative molecules included p18, p21, p27, and p53. GAPDH was used as an internal control for comparison of expression levels. Each experiment was completed in triplicate and recorded on the graphs as a mean ratio of molecule densitometric Units/GAPDH + SEM (× 100). B Evaluation of CDK4 expression in LNCaP cells via IHC staining. IHC images appear as original magnification of 400X. Relative staining intensity was evaluated via MetaMorph image software analysis of 3–5 randomly selected high-power fields of three slides