Table 6.
Relative mRNA and miRNA expression of the macrophages with different treatments.
| Gene | The Cells with Different Treatments | ||||
|---|---|---|---|---|---|
| Control | LPS | CH | PCH | PCH-Hyp | |
| iNOS | 1.00 | 2.41 ± 0.04 | 2.29 ± 0.03 | 1.93 ± 0.16 | 1.71 ± 0.12 |
| IL-6 | 1.00 | 2.47 ± 0.05 | 2.28 ± 0.04 | 1.92 ± 0.28 | 1.84 ± 0.09 |
| TNF-α | 1.00 | 1.89 ± 0.08 | 1.81 ± 0.09 | 1.7 3± 0.14 | 1.68 ± 0.03 |
| IL-1β | 1.00 | 2.25 ± 0.13 | 2.19 ± 0.11 | 2.03 ± 0.07 | 1.80 ± 0.08 |
| COX-2 | 1.00 | 2.14 ± 0.03 | 1.97 ± 0.05 | 1.73 ± 0.04 | 1.67 ± 0.05 |
| TLR4 | 1.00 | 1.91 ± 0.02 | 1.86 ± 0.15 | 1.79 ± 0.13 | 1.73 ± 0.07 |
| IL-10 | 1.00 | 1.45 ± 0.02 | 1.53 ± 0.12 | 1.61 ± 0.19 | 1.68 ± 0.12 |
| TGF-β1 | 1.00 | 1.36 ± 0.06 | 1.38 ± 0.07 | 1.47 ± 0.02 | 1.51 ± 0.11 |
| miR-181a | 1.00 | 0.78 ± 0.04 | 1.43 ± 0.08 | 1.51 ± 0.06 | 1.75 ± 0.06 |
| miR-30d | 1.00 | 0.61 ± 0.05 | 0.67 ± 0.04 | 0.74 ± 0.04 | 0.77 ± 0.09 |
| miR-155 | 1.00 | 1.87 ± 0.06 | 1.80 ± 0.04 | 1.66 ± 0.04 | 1.59 ± 0.02 |
| miR-148a | 1.00 | 1.55 ± 0.19 | 1.51 ± 0.03 | 1.45 ± 0.07 | 1.37 ± 0.15 |
The relative expression levels of miR-181a, miR-30d, miR-155, and miR-148a were normalized to U6 snRNA, while those levels of other genes were normalized to β-actin.