Table 2. List of primers used for the S gene amplification of SARS-CoV-2.
| Primer S segment |
Sequence (5’–3’) | Coding Position | Product size (pb) |
|---|---|---|---|
| SP1 sense ASP1antisense |
GTTTGTTTTTCTTGTTTTATT
ACAGTGAAGGATTTCAACGTACAC |
(21551–21574) (22450–22474) |
923pb |
| SP2 sense ASP2 antisense |
CGTGATCTCCCTCAGGGTTTT
TCAGCAATCTTTCCAGTTTGCC |
(22190–22211) (22810–22832) |
620pb |
| SP3 sense ASP3 antisense |
GTAATTAGAGGTGATGAAGTCAGA
ACATAGTGTAGGCAATGATGGA |
(22751–22775) (23621–23643) |
892pb |
| SP4 sense ASP4 antisense |
CTTGGCGTGTTTATTCTACAG
GCTTGTGCATTTTGGTTGACC |
(23445–23466) (24403–24424) |
979pb |
| SP5 sense ASP5 antisense |
AGACTCACTTTCTTCCACAGCA
AGATGATAGCCCTTTCCACA |
(24355–24377) (24699–24719) |
342pb |
| SP6 sense ASP6 antisense |
TTCTGCTAATCTTGCTGCTACT
GTTTATGTGTAATGTAATTTGACTCC |
(24610–24632) (25348–25372) |
766pb |
| SP7 sense# ASP7 antisense |
TAGAGAAAACAACAGAGTT
TGAGGGAGATCACGCACTAA |
(21492–21511) (22184–22204) |
712pb |
| SP8 sense# ASP8 antisense |
TTCTGCTAATCTTGCTGCTACT
CCTTGCTTCAAAGTTACAGTTCCA |
(24610–24632) (25409–25433) |
825pb |
# Set primers 7 and 8 flanks the S protein-coding region.
Nucleotide positions are according to the SARS-CoV-2 Wuhan (Genbank accession no.NC_ 045512-Wuhan-HU-1).