Table 3. Statistical analysis of Ct value distribution per gene region and test system for RM 1 and RM 2.

Additional information can be found in S3 Table.

Gene Region	Test kit	RM 1				RM 2
		N	Mean	Median	+/- 2 SD from mean	N	Mean	Median	+/- 2 SD from mean
E gene	Allplex 2019 n-CoV Assay	33	20.7	20.5	18.0–23.3	33	24.0	23.8	21.3–26.6
	ampliCube Coronavirus SARS-CoV-2	15	22.1	21.9	18.5–25.8	15	25.3	24.7	22.1–28.5
	COBAS SARS-CoV-2 Test	38	22.4	22.3	21.1–23.8	38	25.6	25.5	24.2–26.9
	IN-HOUSE	23	23.4	23.2	18.5–28.3	23	26.6	26.4	22.1–31.1
	LightMix Modular SARS and Wuhan CoV E gene	24	22.7	22.3	18.9–26.5	24	26.0	25.8	22.0–29.9
	LightMix Sarbeco E gene	22	23.3	23.0	19.3–27.3	22	26.4	26.1	22.5–30.4
	RealStar SARS-CoV-2 RT-PCR Kit 1.0	50	21.3	21.5	17.4–25.2	50	24.4	24.6	20.6–28.2
	RIDA GENE SARS-CoV-2	68	22.9	22.3	18.0–27.7	68	26.1	25.6	21.1–31.1
	Xpert Xpress SARS-CoV-2	51	21.4	21.3	20.2–22.7	51	24.5	24.4	23.4–25.7
N gene	Allplex 2019 n-CoV Assay	33	22.7	22.5	20.4–24.9	33	26.0	26.0	23.7–28.2
	Allplex SARS-CoV-2/FluA/FluB/RSV Assay	27	20.9	21.3	18.1–23.8	27	24.0	24.1	21.1–26.9
	BD SARS-CoV-2	33	20.3	20.0	18.3–22.3	33	23.5	23.3	21.0–25.9
	IN-HOUSE	25	22.8	22.4	19.0–26.7	25	26.0	25.7	22.2–29.8
	Xpert Xpress SARS-CoV-2	56	23.4	23.5	22.0–24.9	56	26.6	26.6	25.2–28.1
ORF1ab	COBAS SARS-CoV-2 Test	49	22.2	22.1	20.6–23.7	49	25.3	25.2	23.9–26.6
RdRP gene	Allplex 2019 n-CoV Assay	32	22.6	22.5	19.8–25.4	32	26.0	25.7	23.1–28.9
	Allplex SARS-CoV-2/FluA/FluB/RSV Assay	27	21.6	21.6	17.9–25.4	27	24.8	25.3	21.4–28.3
	IN-HOUSE	17	23.1	22.2	18.4–27.7	17	26.3	25.6	21.6–30.9
	LightMix Modular Wuhan CoV RdRP gene	24	26.3	26.0	21.3–31.3	24	29.8	29.0	24.4–35.1
S gene	Allplex SARS-CoV-2/FluA/FluB/RSV Assay	29	20.8	20.3	17.1–24.5	29	23.9	23.7	20.3–27.5
	RealStar SARS-CoV-2 RT-PCR Kit 1.0	49	21.4	21.3	18.2–24.6	49	24.5	24.4	21.1–27.9