Table 4. Statistical analysis of Ct value distribution per gene region and for fully automated systems and in-house assays for RM 1 and RM 2.

		RM 1				RM 2
Gene Region	System	N	Mean	Median	+/- 2 SD from mean	N	Mean	Median	+/- 2 SD from mean
E gene	fully automated	96	22.1	21.6	18.4–25.8	96	25.2	24.7	21.4–28.9
	in-house	23	23.4	23.2	18.4–28.5	23	26.6	26.4	22.0–31.3
N gene	fully automated	130	22.3	23.1	18.3–26.3	130	25.5	26.3	21.5–29.6
	in-house	25	22.8	22.4	18.9–26.5	25	26.0	25.7	22.1–29.6