Table 2.

Influence of mutations of selected interleukins on their activity.

Name	Mutation Site	Change	Effect	Reference
IL-6	173	A → V	Almost no loss of activity	[51,88]
	185	W → R	No loss of activity
	204	S → P	87% loss of activity
	210	R → K, E, Q, T, A or P	Loss of activity
	212	M → T, N, S or R
IL-17A	69	R → A	Impairs binding to IL-17RA and IL-17RC	[63,89]
	78	R → V	Decreases the affinity for IL-17RA by 5-fold	[63,90]
	90	W → V	Has no effect on the affinity for IL-17RA
	108	Y → I	Decreases the affinity for IL-17RA
	109	H → S
IL-18	40	K → A	Reduces binding to IL-18R1 and the ability to induce IFNG production	[64,91]
	41	L → A	Impairs binding to IL-18R1 and the ability to induce IFNG production
	44	K → A:	Reduces binding to IL-18R1 and the ability to induce IFNG production
	49	R → A
	53	D → A
	69	M → A	Impairs binding to IL-18R1 and the ability to induce IFNG production
	71	D → A
	94	R → A
	96	M → A
	115	K → A	Reduces binding of the preformed binary complex of IL-18 and IL-18R1 to IL-18RAP resulting in impaired IFNG production
	120	K → A
	134	D → A
	140	R → A	Reduces binding to IL-18R1 and the ability to induce IFNG production
	144	G → A	Abolishes binding of the preformed binary complex of IL-18 and IL-18R1 to IL-18RAP
	145	H → A
	146	D → A	Reduces binding of the preformed binary complex of IL-18 and IL-18R1 to IL-18RAP
	148	K → A	Abolishes binding of the preformed binary complex of IL-18 and IL-18R1 to IL-18RAP
	168	D → A	Reduces binding to IL-18R1 and the ability to induce IFNG production
	183	R → A	Reduces binding of the preformed binary complex of IL-18 and IL-18R1 to IL-18RAP
	186	M → A
IL-33	144	E → K	Decreases affinity for IL-1RL1	[80,92]
	148	E → K	7-fold decrease in affinity for IL-1RL1
	149	D → K	Almost abolishes binding to IL-1RL1
	165	E → K	8-fold decrease in affinity for IL-1RL1
	244	D → K	Decreases affinity for IL-1RL1