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. 2022 Jan 8;23(2):676. doi: 10.3390/ijms23020676

Figure 1.

Figure 1

Schematics of the lectin nano-blocks. (A) Design of the lectin nano-blocks. The lectin nano-blocks were constructed by fusing the dimeric de novo protein WA20 (PDB ID: 3VJF) [9] to the dimeric lectin Agrocybe cylindracea galectin (ACG) (PDB ID: 1WW7) [30] with different type of linkers (HL4, FL4, SL, and H). In addition, WA20-ΔN3ACG was constructed by fusing WA20 and ACG without a linker and with the deletion of the N-terminal 3 aa of ACG. Since both WA20 and ACG form dimer, the lectin nano-blocks are expected to form self-assembling oligomers in multiples of 2-mer. (B) Schematics of the binding of the lectin nano-blocks and ACG to target glycans on cells. Because the lectin nano-block oligomers have more carbohydrate recognition domains (CRDs) than the original ACG, they are expected to enhance the binding avidity to target glycans by multivalent binding effect.