Figure 5.

Glycoconjugate microarray analysis. Specificity profiles of rACG, WA20, and the five lectin nano-blocks. The Cy3-labeled proteins (1 µg/mL or 10 µg/mL) were applied to the array, and binding was detected by the scanner multiple times by varying the exposure time (0.1–3 s). The fluorescent intensity was normalized to the intensity per second. Abbreviations: FET, fetuin; AGP, α1-acid glycoprotein; TG, porcine thyroglobulin; TF, transferrin; BSM, bovine submaxillary mucin, and GP; human glycophorin.