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. 2022 Jan 8;23(2):676. doi: 10.3390/ijms23020676

Figure 7.

Figure 7

SPR analysis. Sensorgrams of the fractionated samples (Fr.) of the lectin nano-block oligomers of (A) WA20-H-ACG and (B) WA20-ΔN3ACG at 1 µM bound to the 3′-sialyllactose ligand. The sensorgram of rACG at 1 µM is also shown for comparison. Sensorgrams of all samples at all concentrations are shown in Figures S13 and S14. Bold lines show the fitting curves used for calculating the apparent dissociation rate constants kd_app_early in the early phase (181–211 s) and kd_app_late in the late phase (480–780 s) of the lectin nano-block oligomers. The apparent dissociation rate constant kd_app of rACG was calculated using the data in the entire dissociation time (181–780 s).