Fig 1. Multi-spectral camera system for the mouse vision.
(A) Schematic diagram of the camera optics. Incoming light was split into UV and Green channels by a dichroic mirror and further filtered to match the spectral sensitivity of the mouse visual system (see panel B). A neutral density filter with the optical density value from 1.0 to 2.0 was used for the Green channel to maximize the dynamic range of the camera sensor to be used with the same parameter settings as the UV channel. The inset shows the pixel intensity values as a function of the exposure time (mean ± standard deviation; N = 2,304,000 pixels), supporting the linearity of the camera sensor (Sony, IMX174 CMOS). (B) Relative spectral sensitivity of the camera system (UV channel, violet area; Green channel, green area). For comparison, the spectral sensitivity of the mouse rod and S- and M-cone photoreceptors [31] corrected with the transmission spectrum of the mouse eye optics [30] was shown in black, violet and green lines, respectively, as well as typical sunlight spectrum in gray.
