Figure 1.

Schematic diagram of this study. For the screening of the protein targets of penetratin, biotinylated penetratin was probed on yeast proteome microarrays followed by streptavidin-DyLight 650 and anti-His antibody-DyLight 550. For simplicity, His tag and anti-His antibody signal of the yeast protein are not shown. The yeast proteome microarrays were scanned and the interacting signals of penentratin with yeast proteins partners were obtained. Data from triplicate yeast proteome microarrays assays of penetratin were statistically analyzed following cutoff parameters to identify the protein targets of penetratin. These identified protein targets were subjected to several bioinformatics tools, like PANTHER, DAVID, REVIGO, STRING, and STREME databases to understand the binding pattern through protein classification, enrichment in gene ontology, protein–protein interaction network, and enriched motif.