High-molecular-weight and degraded eDNA released from 5-day-old dehydrated Pseudomonas aeruginosa ATCC 27853 (PA) and Staphylococcus aureus ATCC 25923 (SA) biofilms after treatment with biocides. Shown here are 1.2% agarose-TA gels stained with EtBr to visualize the eDNA released from PA (left) and SA (right) biofilms after 1 h of exposure to biocides. Treatments: (1) water-only control, (2) 0.1% PHMG-Cl, (3) 0.05% PHMG-Cl, (4) 6% hydrogen peroxide, (5) 3% hydrogen peroxide, (6) 70% ethanol, (7) 0.05% chlorhexidine, (8) 0.25% Maxisan, (9) Arquades-plus, (10) Desmanol, and (11) Sanikon. A sample of GeneRuler 1 kb DNA Ladder (Thermo Fisher Scientific, Vilnius, Lithuania) is shown in the rightmost lane of each gel (12).