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. 2022 Jan 10;23(2):731. doi: 10.3390/ijms23020731

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Metabolic assays show the biocidal activity of PHMG-Cl on Pseudomonas aeruginosa ATCC 27853 (PA) and Staphylococcus aureus ATCC 25923 (SA) biofilms growing in situ. Shown here are the metabolic activity of 24 h PA (left) and SA (right) biofilms after treatment with PHMG-Cl for 1 h (n = 8). Statistical differences are determined between treatments and the water-treated (live) positive controls (a) and the EtOH-treated (dead) negative controls (b); * p < 0.05, ** p < 0.01, and *** p < 0.005.