Table 2.
Wolbachia MLST profiles of the analysed Acrididae isolates.
| Isolate | Species (Region, Year) | Supergroup | gatB | coxA | hcpA | ftsZ | fbpA | Sequ-Ence Type * |
|---|---|---|---|---|---|---|---|---|
| i-1 | Arcyptera fusca (Altai, 2017) | F | ~73 ** (MZ816480) | ~63 (MZ816523) | ~261 (MZ816567) | No *** | 410 (MZ816669) | not full |
| i-2 | Chorthippus albomarginatus (Irkutsk, 2016) | B | 134 (MZ816445) | 168 (MZ816488) | ~6 (MZ816532) | 106 (MZ816581) | 197 (MZ816634) | hST-1 |
| i-3 | Ch. Apricarius (Irkutsk, 2016) | B | 134 (MZ816446) | 14 (MZ816489) | ~6 (MZ816533) | 106 (MZ816582) | 197 (MZ816635) | hST-2 |
| i-4 | Ch. Biguttulus (Irkutsk, 2016) | B | 9 (MZ816447) | 133 (MZ816490) | ~6 (MZ816534) | 106 (MZ816583) | 197 (MZ816636) | hST-3 |
| i-5 | Ch. Biguttulus (Novosibirsk, 2017) | B | 9 (MZ816448) | 133 (MZ816491) | 6 (MZ816535) | 106 (MZ816584) | 197 (MZ816637) | hST-4 |
| i-6 | Ch. Biguttulus (Altai, 2015) | B | 9 (MZ816481) | 133 (MZ816524) | ? **** | ? | ? | not full |
| i-7 | Ch. Mollis (Altai, 2003) | B | 9 (MZ816449) | 133 (MZ816492) | 6 (MZ816536) | 106 (MZ816585) | 197 (MZ816638) | hST-4 |
| i-8 | Ch. Fallax (Novosibirsk, 2017) | B | 9 (MZ816450) | 133 (MZ816493) | 6 (MZ816537) | 106 (MZ816586) | 197 (MZ816639) | hST-4 |
| i-9 | Dociostaurus brevicollis (Kazakhstan, 2007) | F | ~73 (MZ816451) | ~63 (MZ816494) | ~261 (MZ816538) | ~269 (MZ816587) | 410 (MZ816640) | hST-5 |
| i-10 | Eclipophleps glacialis (G, Altai, 2003) | B | 9 (MZ816482) | 133 (MZ816525) | ~6R (MZ816568) | No | ~4 (MZ816670) | not full |
| i-11 | Gomphocerippus rufus (G, Novosibirsk, 2017) | B | 9 (MZ816452) | 133 (MZ816495) | 6 (MZ816539) | 106 (MZ816588) | 197 (MZ816641) | hST-4 |
| i-12 | Omocestus viridulus (Novosibirsk, 2009) | B | 9 (MZ816453) | 133 (MZ816496) | 6 (MZ816540) | 106 (MZ816589) | 197 (MZ816642) | hST-4 |
| i-13 | Om. Viridulus (Altai, 2017) | B | 134 (MZ816454) | 168 (MZ816497) | ~6 (MZ816541) | 106 (MZ816590) | 197 (MZ816643) | hST-1 |
| i-14 | Om. Haemorrhoidalis (Altai, 2017) | B | 134 (MZ816455) | 14 (MZ816498) | ~6 (MZ816542) | 106 (MZ816591) | 197 (MZ816644) | hST-2 |
| i-15 | Podismopsis altaica (Altai, 2003) | F | No | ~63 (MZ816526) | ~325 (MZ816569) | ? | ? | not full |
| i-16 | Podismopsis genicularibus (Sakhalin Is., 2010) | B–A | 134 (MZ816456) | 14 (MZ816499) | ~6 (MZ816543) | 226 (MZ816592) | 197 (MZ816645) | hST-6 |
| i-17 | Pseudochorthippus montanus (Novosibirsk, 2017) | B | 9 (MZ816457) | 133 (MZ816500) | 6 (MZ816544) | 106 (MZ816593) | 197 (MZ816646) | hST-4 |
| i-18 | Ps. Montanus (Novosibirsk, 2017) | B | 9 (MZ816483) | 133 (MZ816527) | 6 (MZ816570) | ? | ? | not full |
| i-19 | Ps. Parallelus (Novosibirsk, 2017) | B | 9 (MZ816458) | 133 (MZ816501) | 6 (MZ816545) | 106 (MZ816594) | 197 (MZ816647) | hST-4 |
| i-20 | Stauroderus scalaris (Altai, 2017) | B | 9 (MZ816459) | 133 (MZ816502) | 6 (MZ816546) | 106 (MZ816595) | 197 (MZ816648) | hST-4 |
| i-21 | Bryodema gebleri (Altai, 2003) | F | ~73 (MZ816460) | ~63 (MZ816503) | ~261 (MZ816547) | ~205 (MZ816596) | 410 (MZ816649) | hST-7 |
| i-22 | Bryodema gebleri (Altai, 2017) | F | ~73 (MZ816461) | ~63 (MZ816504) | ~261 (MZ816548) | ~205 (MZ816597) | 410 (MZ816650) | hST-7 |
| i-23 | Oedaleus decorus (Altai, 2017) | F | ~73 (MZ816462) | ~63 (MZ816505) | ~35 (MZ816549) | ~205 (MZ816598) | 410 (MZ816651) | hST-8 |
| i-24 | Oe. Decorus (Kazakhstan, 2006) | F | ~243 (MZ816484) | ~63RK (MZ816528) | ~261 (MZ816571) | ~205R (MZ816616) | 410 (MZ816671) | N, full |
| i-25 | Oe. Decorus (Tajikistan, 2009) | F | ~243Y (MZ816485) | ~30YR (MZ816529) | ~261Y (MZ816572) | ~205 (MZ816617) | 410 (MZ816672) | N, full |
| i-26 | Anapodisma miramae (Far East, Russia, 2008 ) | B | 39 (MZ816463) | 14 (MZ816506) | 40 (MZ816550) | 7 (MZ816599) | 197 (MZ816652) | ST299 |
| i-27 | Bohemanella frigida (Altai 2003) | B | 9 (MZ816464) | 9 (MZ816507) | 6 (MZ816551) | 106 (MZ816600) | 197 (MZ816653) | hST-9 |
| i-28 | Ognevia longipennis (Japan, 2005) | B | 9 (MZ816465) | 133 (MZ816508) | 6 (MZ816552) | 106 (MZ816601) | 197 (MZ816654) | hST-4 |
| i-29 | Og. Longipennis (Altai, 2003) | B | 9 (MZ816466) | 133 (MZ816509) | 6 (MZ816553) | 106 (MZ816602) | 197 (MZ816655) | hST-4 |
| i-30 | Podisma pedestris (Altai, 2003) | B | 9 (MZ816467) | 133 (MZ816510) | 6 (MZ816554) | 106 (MZ816603) | 197 (MZ816656) | hST-4 |
| i-31 | P. pedestris (Altai, 2016) | B | 9 (MZ816468) | 133 (MZ816511) | 6 (MZ816555) | 106 (MZ816604) | 197 (MZ816657) | hST-4 |
| i-32 | Prumna littoralis (Far East, Russia, 2008) | B | 9 (MZ816469) | 133 (MZ816512) | 6 (MZ816556) | 106 (MZ816605) | 197 (MZ816658) | hST-4 |
| i-33 | Pr. Primnoa (Sakhalin, 2010) | B | 9 (MZ816470) | 133 (MZ816513) | 6 (MZ816557) | 106 (MZ816606) | 197 (MZ816659) | hST-4 |
| i-34 | Pr. Primnoa (Sakhalin Is., 2010) | B | 188 (MZ816471) | 224 (MZ816514) | ~6 (MZ816558) | 20 (MZ816607) | 25 (MZ816660) | hST-10 |
| i-35 | Pr. Ussuriensis (Far East, Russia, 2008) | B | 9 (MZ816472) | 133 (MZ816515) | 6 (MZ816559) | 106 (MZ816608) | 197 (MZ816661) | hST-4 |
| i-36 | Podisma kanoi (Honshu, Japan, 2005) | B | 9 (MZ816473) | 133 (MZ816516) | 6 (MZ816560) | 106 (MZ816609) | 197 (MZ816662) | hST-4 |
| i-37 | P. sapporensis (Japan, Tanno, 2005) | B | 9 (MZ816474) | 14 (MZ816517) | 6 (MZ816561) | 106 (MZ816610) | 197 (MZ816663) | hST-11 |
| i-38 | P. sapporensis (Japan, Akan, 2005) | B | 9 (MZ816475) | 14 (MZ816518) | 6 (MZ816562) | 106 (MZ816611) | 197 (MZ816664) | hST-11 |
| i-39 | P. sapporensis (Japan, Yotei, 2005) | B | 9 (MZ816476) | 14 (MZ816519) | 6 (MZ816563) | 106 (MZ816612) | 197 (MZ816665) | hST-11 |
| i-40 | P. sapporensis (Japan, Disengen, 2005) | B | 9 (MZ816486) | 14 (MZ816530) | 6 (MZ816573) | Mix ***** (MZ816618-MZ816632) | 197 (MZ816673) | Mix |
| i-41 | P. sapporensis (Japan, Naganuma, 2005) | B | 9 (MZ816477) | 14 (MZ816520) | 6 (MZ816564) | 106 (MZ816613) | 197 (MZ816666) | hST-11 |
| i-42 | P. sapporensis (Japan, Teine, 2005) | B | 9 (MZ816487) | 73 (MZ816531) | Mix (MZ816574-MZ816580) | 106 (MZ816633) | Mix (MZ816674-MZ816686) | Mix |
| i-43 | P. sapporensis (Japan, Shimokawa, 2005) | B | 9 (MZ816478) | 14 (MZ816521) | 6 (MZ816565) | 106 (MZ816614) | 197 (MZ816667) | hST-11 |
| i-44 | P. tyatiensis (Kunashir Is., Russia, 2001) | B | 9 (MZ816479) | 14 (MZ816522) | 6 (MZ816566) | 106 (MZ816615) | 197 (MZ816668) | hST-11 |
* ST numbers according to the PubMLST database or haplotype numbers according to this study or short comments are provided; ** the ‘~ number’ refers to the most closely related alleles according to the PubMLST database; *** no PCR product; **** multiple double chromatogram peaks; ***** mix: a DNA sample that yielded multiple double chromatogram peaks was cloned and sequenced.