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The biological efficacy of hydrogel-released EVs on hBMSCs behaviour. The influence on hydrogel-released EVs on hBMSCs (A) EV uptake (Scale bar = 20 µm), (B) proliferation, (C) migration, (D) H3K9 acetylation levels, (F) collagen production and (E,F) calcium deposition. Black staining indicates mineral nodules. Scale bar = 100 µm. Data are expressed as mean ± SD (n = 3). * p ≤ 0.05, ** p ≤ 0.01 and *** p ≤ 0.001.
