Table 2.
Specific ß-galactosidase activities obtained from the expression of lacZ reporter constructs.
| Plasmid | Promoter | Specific Activity [mU/mg Protein] 1 | |
|---|---|---|---|
| 2% Glucose | 2% Ethanol | ||
| pJJH3043 | KlFBA1p | 224 ± 6 | 162 ± 4 |
| pJJH3061 | KlTDH1p | 118 ± 8 | 363 ± 13 |
| pJJH3042 | KlTDH2p | 127 ± 6 | 222 ± 8 |
| pJJH3041 | KlGDP1p | 3 ± 1 | 22 ± 1 |
| pJJH2031 | none | <1 | <1 |
1 Strains were grown after inoculation from a preculture for 6 h in logarithmic phase on selective synthetic medium lacking uracil with the indicated carbon sources. Specific ß-galactosidase activities were measured twice in crude extracts from two independent transformants, each. Standard deviations are given (±). KHO209-8A was used as a recipient strain for all constructs. It lacks intrinsic ß-galactosidase activity due to a deletion of the KlLAC4 gene.