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. 2022 Jan 6;23(2):589. doi: 10.3390/ijms23020589

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Knockdown of G9a mitigates the aggressiveness of breast cancer cells. (A) Expression of G9a in shRNA against G9a (shG9a) or non-silencing control (NS)-expressed breast cancer cells was evaluated by Western blot or qPCR. (B) G9a-depleted cell lines showed low migration and invasion abilities. (C) G9a-depleted cell lines showed increase in epithelial marker expressions. (D) In vivo study revealed reduced tumorigenesis following G9a-knockdown cell-line injection. (E) Sphere-forming ability was diminished by G9a depletion in MCF7 cells. (F) G9a downregulation enhanced the efficacy of ionizing radiation. (G) G9a knockdown augmented apoptotic cell death. p values were calculated using ANOVA. ** p < 0.01, *** p < 0.001, **** p < 0.0001.