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. 2022 Jan 6;23(2):593. doi: 10.3390/ijms23020593

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Principal component analysis (PCA), Venn diagram, and heatmap from RNA-seq profile. (A) A PCA plot for HeLa-NT, XRCC4 knockout, mirin-treated, and XRCC4 knockout + mirin-treated samples from whole-transcriptome RNA-seq data using ClusVis [32]. (B) A Venn diagram representing shared and unique differentially expressed genes (DEGs) across three categories: (i) XRCC4 knockout cells (blue) (ii) mirin treated (green) (iii) XRCC4 knockout with mirin treated (red) when compared to HeLa-NT cells. (C) A heatmap of top DEGs across each sample categories. Knockout = KO. The analyses are based on duplicate experiments.