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. 2022 Jan 12;23(2):810. doi: 10.3390/ijms23020810

Figure 2.

Figure 2

Effect of EA on iNOS expression and NO production in BV-2 cells treated with LPS and IFN-γ. BV-2 cells were treated with the indicated concentration of EA for 15 min and then stimulated with LPS (500 ng/mL) and IFN-γ (50 ng/mL) for 24 h. (A) The protein levels of iNOS were detected by western blot (n = 3). β-actin was used as a loading control. (B) Quantitative densitometry was used to calculate the ratio of iNOS to β-actin, which was normalized to the control. The relative density was estimated by NIH ImageJ densitometric analysis. (C) Estimated NO release derived from the nitrite present in the culture medium using the Griess reagent assay (n = 3). The results are expressed as the mean ± SD. * p < 0.05 compared to the untreated group. # p < 0.05 compared to the LPS/IFN-γ groups.