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Steady state kinetic parameters of M. smegmatis DprE1 and IC50 values of selamectin. SEL, selamectin; nd, not determined.
| DprE1 Variant |
Km (µM) |
kcat (min−1) |
kcat/Km (min−1 µM−1) |
IC50 SEL (µM) |
|---|---|---|---|---|
| Wild-type | 0.19 ± 0.20 | 5.1 ± 0.4 | 26.8 ± 1.1 | 2.6 ± 1.1 |
| C394A | 0.33 ± 0.22 | 2.8 ± 0.5 | 8.5 ± 0.6 | 3.9 ± 1.0 |
| C394G | nd | nd | nd | nd |
| C394S | 0.31 ± 0.15 | 1.6 ± 0.3 | 5.2 ± 0.6 | 12.1 ± 1.2 |
| L282F | 0.16 ± 0.12 | 4.5 ± 0.3 | 28.1 ± 1.4 | 1.0 ± 0.7 |
| L282V | 0.21 ± 0.15 | 4.3 ± 0.4 | 20.4 ± 1.1 | 50.6 ± 9.2 |
