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. Author manuscript; available in PMC: 2022 Jan 20.
Published in final edited form as: Cancer Res. 2021 Apr 14;81(12):3295–3308. doi: 10.1158/0008-5472.CAN-20-3564

Figure 3. Heightened levels of ELR+ CXC chemokines in preclinical LKB1-deficient NSCLC tumors are associated with a G-MDSC-enriched microenvironment.

Figure 3.

A) Relative gene expression of the indicated chemokines in multiple lung tumors from K, KP, and KL genetically-engineered mice was determined by q-PCR. Data are presented as a heatmap. B) The overall scores of the indicated chemokine expression in KL, KP, and K tumors are presented. C) CXCL1 concentration in bronchoalveolar lavage (BAL) fluid from KP and KL mice 4 weeks (4w) post tumor induction was evaluated by ELISA. Four mice per group were pooled and subjected to analysis. D) Heatmap of relative gene expression of the indicated chemokines in the syngeneic murine NSCLC cell lines (left). The overall scores of ELR+ CXC chemokines from KPL and KP murine NSCLC cell lines (right). E) The abundance of G-MDSCs in lung tumors of KP and KL mice was determined by flow cytometry. G-MDSC is defined as MHCIIloCD11b+Ly6CloLy6G+. F) The overall ELR+ CXC chemokine scores of three KPL and two KP murine NSCLC cell lines. G) The abundance of G-MDSC within subcutaneous tumors derived from the same cells as in F (left), [1940A (1×105), 1950A (3×105), 1942B (1×106), 1969B (1×106), 2042 (1.5×106) cells in FBV mice]. The correlation between the overall score and G-MDSC percentage in KPL and KP tumors (p=0.027) (right). H) The abundance of G-MDSC in the blood of mice bearing subcutaneous KPL and KP tumors on day 9 and day 14. I) The abundance of G-MDSC in the spleen of mice bearing subcutaneous KPL and KP tumors on day 15 as in G. * P<0.05; **, P< 0.01; ***, P<0.001; ****, P< 0.0001. n.s., not significant.