Fig. 2.

Schematic workflow of in silico designing a multi-epitope vaccine. A. Collecting the required information regarding the potential vaccine target structures B. Predicting the potential T-cell and B-cell and IFN-γ epitopes in the structure of the molecular target, such as spike protein C. Joining the selected epitopes, and peptide adjuvants by using suitable linkers D. structure prediction and analyzing the modeled multi-epitope peptide E. Molecular docking with potential immune receptors, such as TLRs F. Molecular dynamics simulation to investigate the stability of the complex G.In silico simulation of immune response toward the designed multi-epitope construct.